The players, based out of The Crispin pub in Wokingham, will try to score 500,001 points in a 48-hour event involving its A and B teams, with the target of landing the final dart on a double.
Starting at 8pm on August 1, organisers hope to spur on donations in aid of Diabetes UK, The Stroke Association and VICTA (Visually Impaired Children Taking Action).
Though the team has done similar events before this year they decided to set the bar higher.
Event organiser Steve Taylor said: “We’ve walked the distance of a marathon by playing darts before and wanted to do something different.
“We’ve set ourselves quite a challenge!”
On the night the 16 players, split into two teams, will do their best to stave off sleep and keep their eyes on the prize.
Though the half million mark may seem a tall ask the effort was nearly bigger. David Steatham, captain of the B-team, said: “We were thinking about trying to do a million points, though that seemed a little far-fetched.”
The players have been testing whether they can reach their mark in time and so far they look set to make it.
Mr Steatham said: “We have done some 10 to 30-minute sections of play to see if we can get to the end in 48 hours and we are on target to throw within the time.
“It is just a question of if we can keep up that pace for two days.”
Time and stamina will be the main obstacles though Mr Steatham admitted that they have not exactly worked on endurance training.
He added: “Hopefully I will be able to stay up for the whole thing.
“I don’t want to crash out on a sofa halfway through.”
To donate, visit www.virginmoneygiving.com/crispindartschallenge